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Cell Signaling Technology Inc phosphorylated p perk

Daphnetin suppresses endoplasmic reticulum (ER) stress-induced apoptosis in diabetic rats. (A) The expression levels of GRP78, CHOP, <t>p-PERK,</t> and PERK were examined by western blotting. The ratio of p-PERK/PERK was calculated. GAPDH was utilized as the internal control. (B) GRP78 and CHOP expression levels were quantified by immunofluorescence staining. (C) The number of apoptotic cells in myocardial tissues was determined by TUNEL assay. (D) The levels of Bax, Bcl-2, and cleaved caspase-3 in myocardial tissues were examined by western blotting. GAPDH was utilized as the internal control. ## indicates P <0.01 compared to the control group. * indicates P <0.05 and ** indicates P <0.01 compared to the STZ group.

Phosphorylated P Perk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 934 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorylated+p+perk/Phospho-PERK+(Thr980)+Rabbit+mAb/pmc11742473-70-61-65
Average 96 stars, based on 934 article reviews

phosphorylated p perk - by Bioz Stars, 2026-09

96/100 stars

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1) Product Images from "Daphnetin ameliorates diabetic cardiomyopathy by regulating inflammation and endoplasmic reticulum stress-induced apoptosis"

Article Title: Daphnetin ameliorates diabetic cardiomyopathy by regulating inflammation and endoplasmic reticulum stress-induced apoptosis

Journal: Experimental Animals

doi: 10.1538/expanim.24-0027

Daphnetin suppresses endoplasmic reticulum (ER) stress-induced apoptosis in diabetic rats. (A) The expression levels of GRP78, CHOP, p-PERK, and PERK were examined by western blotting. The ratio of p-PERK/PERK was calculated. GAPDH was utilized as the internal control. (B) GRP78 and CHOP expression levels were quantified by immunofluorescence staining. (C) The number of apoptotic cells in myocardial tissues was determined by TUNEL assay. (D) The levels of Bax, Bcl-2, and cleaved caspase-3 in myocardial tissues were examined by western blotting. GAPDH was utilized as the internal control. ## indicates P <0.01 compared to the control group. * indicates P <0.05 and ** indicates P <0.01 compared to the STZ group.

Figure Legend Snippet: Daphnetin suppresses endoplasmic reticulum (ER) stress-induced apoptosis in diabetic rats. (A) The expression levels of GRP78, CHOP, p-PERK, and PERK were examined by western blotting. The ratio of p-PERK/PERK was calculated. GAPDH was utilized as the internal control. (B) GRP78 and CHOP expression levels were quantified by immunofluorescence staining. (C) The number of apoptotic cells in myocardial tissues was determined by TUNEL assay. (D) The levels of Bax, Bcl-2, and cleaved caspase-3 in myocardial tissues were examined by western blotting. GAPDH was utilized as the internal control. ## indicates P <0.01 compared to the control group. * indicates P <0.05 and ** indicates P <0.01 compared to the STZ group.

Techniques Used: Expressing, Western Blot, Control, Immunofluorescence, Staining, TUNEL Assay

Incubation:

Article Title: Ginsenoside Rg1 regulates autophagy and endoplasmic reticulum stress via the AMPK/mTOR and PERK/ATF4/CHOP pathways to alleviate alcohol‑induced myocardial injury.
Article Snippet: .. Subsequently, the membranes were blocked with 5% skimmed milk at room temperature for 1.5 h and were then incubated with primary antibodies against Bcl‐2 associated X‐protein (Bax; cat. no. ab32503; 1/1,000; Abcam), B‐cell lymphoma 2 (Bcl‐2; cat. no. ab196495; 1/1,000; Abcam), light chain 3 (LC3)‐II/I (cat. no. ab192890; 1/1,000; Abcam), Beclin‐1 (cat. no. ab207612; 1/1,000; Abcam), p62 (cat. no. ab109012; 1/10,000; Abcam), phosphorylated (p)‐PERK (cat. no. #3179; 1/1,000; Cell Signaling Technology), PERK (cat. no. ab229912; 1/1,000; Abcam), p‐eIF2α (cat. no. #3398; 1/1,000; Cell Signaling Technology), eIF2α (cat. no. #5324; 1/1,000; Cell Signaling Technology), activating transcription factor 4 (ATF4; cat. no. #11815; 1/1,000; Cell Signaling Technology), CHOP (cat. no. 15204‐1‐AP; 1/1,000; Proteintech Group, Inc.), caspase‐12 (cat. no. ab62484; 1/1,000; Abcam), p‐AMPK (cat. no. ab133448; 1/1,000; Abcam), AMPK (cat. no. ab207442; 1/1,000; Abcam), p‐mammalian target of rapamycin (mTOR; cat. no. ab109268; 1/1,000; Abcam), mTOR (cat. no. ab134903; 1/10,000; Abcam) and GAPDH (cat. no. ab181602; 1/10,000; Abcam) at 4 ̊C overnight. .. Following washing three times with PBS/Tween‐20, the membranes were incubated with the corresponding anti‐rabbit horseradish peroxidase‐conjugated secondary antibody (cat. no. ab6721; 1/2,000; Abcam) at room temperature for 1 h. The bands were visualized using an EcL detection kit and their intensity was analyzed using ImageJ 1.8.0 software (National Institutes of Health).

Article Title: Apelin‑13 reduces high glucose‑induced mitochondrial dysfunction in cochlear hair cells by inhibiting endoplasmic reticulum stress.
Article Snippet: .. Protein samples were then transferred to PVDF membranes (MilliporeSigma) and the membranes were blocked with 5% BSA (Sigma‐Aldrich; Merck KGaA) at room temperature for 1 h. Diluted primary antibodies against CHOP (cat. no. 15204‐1‐AP; 1:1,000; Proteintech Group, Inc.), GRP78 (cat. no. 11587‐1‐AP; 1:5,000; Proteintech Group, Inc.), XBP1 (cat. no. 24168‐1‐AP; 1:1,000; Proteintech Group, Inc.), phosphorylated (p)‐PERK (cat. no. 3179; 1:1,000; Cell Signaling Technology, Inc.), PERK (3192; 1:1,000; Cell Signaling Technology, Inc.) and β‐actin (cat. no. 81115‐1‐RR; 1:10,000; Proteintech Group, Inc.) were used to incubate the membranes at 4 ̊C overnight, followed by incubation with a HRP‐conjugated secondary antibody (cat. no. SA00001‐2; 1:5,000; Proteintech Group, Inc.) at 37 ̊C for 1 h. The ECL solution (Vazyme Biotech Co., Ltd.) was then added to evenly cover the membranes for 1 min, and densitometric analysis was performed using the Image‐Pro Plus 7.0 software (Media Cybernetics, Inc.). ..

Article Title: Ginsenoside Rg1 regulates autophagy and endoplasmic reticulum stress via the AMPK/mTOR and PERK/ATF4/CHOP pathways to alleviate alcohol-induced myocardial injury
Article Snippet: .. Subsequently, the membranes were blocked with 5% skimmed milk at room temperature for 1.5 h and were then incubated with primary antibodies against Bcl-2 associated X-protein (Bax; cat. no. ab32503; 1/1,000; Abcam), B-cell lymphoma 2 (Bcl-2; cat. no. ab196495; 1/1,000; Abcam), light chain 3 (LC3)-II/I (cat. no. ab192890; 1/1,000; Abcam), Beclin-1 (cat. no. ab207612; 1/1,000; Abcam), p62 (cat. no. ab109012; 1/10,000; Abcam), phosphorylated (p)-PERK (cat. no. #3179; 1/1,000; Cell Signaling Technology), PERK (cat. no. ab229912; 1/1,000; Abcam), p-eIF2α (cat. no. #3398; 1/1,000; Cell Signaling Technology), eIF2α (cat. no. #5324; 1/1,000; Cell Signaling Technology), activating transcription factor 4 (ATF4; cat. no. #11815; 1/1,000; Cell Signaling Technology), CHOP (cat. no. 15204-1-AP; 1/1,000; Proteintech Group, Inc.), caspase-12 (cat. no. ab62484; 1/1,000; Abcam), p-AMPK (cat. no. ab133448; 1/1,000; Abcam), AMPK (cat. no. ab207442; 1/1,000; Abcam), p-mammalian target of rapamycin (mTOR; cat. no. ab109268; 1/1,000; Abcam), mTOR (cat. no. ab134903; 1/10,000; Abcam) and GAPDH (cat. no. ab181602; 1/10,000; Abcam) at 4°C overnight. .. Following washing three times with PBS/Tween-20, the membranes were incubated with the corresponding anti-rabbit horseradish peroxidase-conjugated secondary antibody (cat. no. ab6721; 1/2,000; Abcam) at room temperature for 1 h. The bands were visualized using an ECL detection kit and their intensity was analyzed using ImageJ 1.8.0 software (National Institutes of Health).

Article Title: Apelin‑13 reduces high glucose‑induced mitochondrial dysfunction in cochlear hair cells by inhibiting endoplasmic reticulum stress
Article Snippet: .. Protein samples were then transferred to PVDF membranes (MilliporeSigma) and the membranes were blocked with 5% BSA (Sigma-Aldrich; Merck KGaA) at room temperature for 1 h. Diluted primary antibodies against CHOP (cat. no. 15204-1-AP; 1:1,000; Proteintech Group, Inc.), GRP78 (cat. no. 11587-1-AP; 1:5,000; Proteintech Group, Inc.), XBP1 (cat. no. 24168-1-AP; 1:1,000; Proteintech Group, Inc.), phosphorylated (p)-PERK (cat. no. 3179; 1:1,000; Cell Signaling Technology, Inc.), PERK (3192; 1:1,000; Cell Signaling Technology, Inc.) and β-actin (cat. no. 81115-1-RR; 1:10,000; Proteintech Group, Inc.) were used to incubate the membranes at 4 ̊C overnight, followed by incubation with a HRP-conjugated secondary antibody (cat. no. SA00001-2; 1:5,000; Proteintech Group, Inc.) at 37 ̊C for 1 h. The ECL solution (Vazyme Biotech Co., Ltd.) was then added to evenly cover the membranes for 1 min, and densitometric analysis was performed using the Image-Pro Plus 7.0 software (Media Cybernetics, Inc.). .. Data are presented as the mean ± SD of three independent experiments, and data analysis was performed using SPSS 25.0 software (IBM Corp.).

Binding Assay:

Article Title: Milk-derived hexapeptide PGPIPN prevents and attenuates acute alcoholic liver injury in mice by reducing endoplasmic reticulum stress
Article Snippet: The 78 kDa glucose-regulated protein (GRP78; cat. no. ab108615), C/EBP homologous protein (CHOP; cat. no. ab11419), caspase-3 (cat. no. ab4051), cleaved caspase-3 (cat. no. ab49822) and β-actin (cat. no. ab8227) antibodies were purchased from Abcam. .. Protein kinase R-like (PKR) endoplasmic reticulum kinase (PERK; cat. no. 3192), phosphorylated (p)-PERK (Thr980; cat. no. 3179), p-eukaryotic initiation factor 2α (p-eIF-2α; Ser51; cat. no. 3398), eIF-2α (cat. no. 5324), inositol-requiring enzyme 1α (IRE-1α; cat. no. 3294) and spliced X-box binding protein 1 (XBP-1s; cat. no. 82914) antibodies were purchased from Cell Signaling Technology, Inc. .. Secondary antibodies [horseradish peroxidase (HRP)-conjugated goat anti-mouse immunoglobulin G (IgG), cat. no. GAM-HRP; HRP-conjugated goat anti-rabbit IgG, cat. no. GAR-HRP] and Super Signal West Pico kit (ECL Chromogenic kit) was purchased from Thermo Fisher Scientific, Inc.

Software:

Blocking Assay:

Article Title: Daphnetin ameliorates diabetic cardiomyopathy by regulating inflammation and endoplasmic reticulum stress-induced apoptosis
Article Snippet: The protein samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred onto polyvinylidene fluoride membranes (Millipore, Billerica, MA, USA). .. After blocking, the membranes were probed with antibodies against collagen I (#ab270993; 1:1,000; Abcam, Cambridge, MA, USA), collagen III (#ab184993; 1:1,000; Abcam), B cell lymphoma-2 (Bcl-2; #26593-1-AP; 1:3,000; Proteintech), Bcl-2-associated X protein (Bax; #2772; 1:1,000; CST, USA), cleaved caspase-3 (#9661; 1:1,000; CST, Shanghai, China), GRP78 (#11587-1-AP; 1:2,000; Proteintech), CHOP (#15204-1-AP; 1:1,500; Proteintech), protein kinase R-like ER kinase (PERK; #3192; 1:1,000; CST), phosphorylated (p)-PERK (#3179; 1:1,000; CST), c-Jun N-terminal kinase (JNK; #17572-1-AP; 1:3,000; Proteintech), p-JNK (#4668; 1:1,000; CST), p38 mitogen-activated protein kinase (p38 MAPK; #9212; 1:1,000; CST), p-p38 MAPK (#28796-1-AP; 1:1,000; Proteintech). ..

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